Subsequently, cells with foreign or self-mutated pMHC will be distinguished and eliminated by T cells ( 2, 3). The binding will result in changes in the CD3 molecule complex and initiate downstream signaling pathways, which are called TCR triggering. T cells recognize antigens through their TCRs binding to antigen-presenting cells (APCs) surface peptide-major histocompatibility complex (pMHC) ( 1). We further discuss the challenges and potential of using TCR sequencing-based assays to profile alloreactive TCR repertoire as the fingerprint for immune monitoring and prediction of rejection and tolerance. Here, we review the recent advances in TCR sequencing techniques and computational tools, as well as the recent discovery in overall TCR profile and antigen-specific T cells tracking in transplantation.
#T cell receptor repertoire analysis full
Tailing T cell transcriptomes and TCR sequences at the single cell level provides a full landscape of alloreactive T-cell clones development and biofunction in alloresponse. Next-generation sequencing (NGS) techniques provide powerful tools for deep and high-throughput TCR profiling, and facilitate to depict the entire T cell repertoire profile and trace antigen-specific T cells in circulation and local tissues. The unique T-cell receptor (TCR) expressed on the surface of each T cell determines the antigen specificity of the cell and can be the specific fingerprint for identifying and monitoring. T cells play a key role in determining allograft function by mediating allogeneic immune responses to cause rejection, and recent work pointed their role in mediating tolerance in transplantation. Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Changchun, China.Guangyao Tian, Mingqian Li * and Guoyue Lv *
0 kommentar(er)
